Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letters to Editor
  • Published:

Banding in Human Chromosomes treated with Trypsin

Nature New Biology volume 235, pages 52–54 (1972)Cite this article

Abstract

THE differential staining properties of the Giemsa stain were first observed by Pardue and Gall1. They were studying in situ hybridization between mouse satellite DNA and mouse chromosomes, and observed that following certain pretreatment the centromeric regions of mouse chromosomes were more densely stained by Giemsa stain than other regions. The darkly stained regions were considered to consist of constitutive heterochromatin. Similar observations were later made on human chromosomes by Arrighi and Hsu2 and Gagné et al.3. Through modifications of the original methods used in the DNA hybridization work, techniques have been developed which make each chromosome identifiable4–6.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Pardue, M. L., and Gall, J. A., Science, 168, 1356 (1970).

    Article  CAS  Google Scholar 

  2. Arrighi, F. E., and Hsu, T. C., Cytogenetics, 10, 81 (1971).

    Article  CAS  Google Scholar 

  3. Gagné, R., Tanguay, R., and Laberge, C., Nature New Biology, 232, 29 (1971).

    Article  Google Scholar 

  4. Drets, M. E., and Shaw, M. W., Proc. US Nat. Acad. Sci., 68, 2073 (1971).

    Article  CAS  Google Scholar 

  5. Sumner, A. T., Evans, H. J., and Bucklane, R. A., Nature New Biology, 232, 31 (1971).

    Article  CAS  Google Scholar 

  6. Patil, S. R., Merrick, S., and Lubs, H. A., Science, 173, 821 (1971).

    Article  CAS  Google Scholar 

  7. Caspersson, T., Zech, L., and Johansson, J. W., Exp. Cell. Res., 62, 490 (1970).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, University of Saskatchewan, Saskatoon, Saskatchewan

    H. C. WANG & S. FEDOROFF

Authors
  1. H. C. WANG
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. FEDOROFF
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

WANG, H., FEDOROFF, S. Banding in Human Chromosomes treated with Trypsin. Nature New Biology 235, 52–54 (1972). https://doi.org/10.1038/newbio235052a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio235052a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing