Recent technological advances in mass spectrometry promise to add single-cell proteomics to the biologist’s toolbox. Here we discuss the current status and what is needed for this exciting technology to lead to biological insight — alone or as a complement to other omics technologies.
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Spatial single-cell mass spectrometry defines zonation of the hepatocyte proteome
Nature Methods Open Access 02 October 2023
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Acknowledgements
We thank the members of our single-cell and DVP teams in Munich and Copenhagen for discussions. The authors are supported by grants from the Max Planck Society for the Advancement of Science, Germany, and the European Union’s Horizon 2020 research and innovation program ISLET (No. 874839). F.A.R. is an EMBO postdoctoral fellowship holder (ALTF 399-2021). We thank Juliet Percival (Percival Press, Durham, UK) for the scientific illustrations.
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M.M., M.T. and F.A.R. conceived the original idea. M.M., F.A.R. and M.T. wrote and edited the original draft. F.A.R., M.T. and M.M. conceptualized and guided the illustration process. All authors wrote, edited and gave final approval to the manuscript.
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M.M. is an indirect shareholder in Evosep Biosystems. The remaining authors declare no competing interests.
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Rosenberger, F.A., Thielert, M. & Mann, M. Making single-cell proteomics biologically relevant. Nat Methods 20, 320–323 (2023). https://doi.org/10.1038/s41592-023-01771-9
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DOI: https://doi.org/10.1038/s41592-023-01771-9
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